FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000828801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000828801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31889533
Sequences flagged as poor quality0
Sequence length30
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTATCTCGTATG329826910.34279492271022No Hit
TGAGATGAAGCACTGTAGCTCTATCTCGTA27817548.723094188930268No Hit
ATCTCGTATGCCGTCTTCTGCTTGAAAAAA19374916.075633029809499Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGAACCGAATTTGTATCTCGTA9924373.1121089167408003No Hit
TGAGATGAAGCACTGTAGCTCATCTCGTAT7014352.1995775228191645No Hit
TGTAAACATCCTCGACTGGAAGATCTCGTA5430011.7027561990324538No Hit
TACAGTACTGTGATAACTGAATCTCGTATG4655571.4599053551521122No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTA4010681.2576791262512372No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTA3936881.2345367365523978No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTA3400871.0664533720202174No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTAT2712340.8505424021104354No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTA2379840.7462762154591602No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATG2321400.7279504532098353No Hit
AACCCGTAGATCCGAACTTGTATCTCGTAT2244830.7039394399409988No Hit
TCCTTCATTCCACCGGAGTCTGATCTCGTA2222400.6969057841016362No Hit
TGAGATGAAGCACTGTAGCTCAATCTCGTA2210870.6932901776893378No Hit
TGAGATGAAGCACTGTAGCTAATCTCGTAT2110500.6618159005338836No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATG2109200.6614082432627659No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTA1937900.6076915582300939No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGT1934880.6067445390310356No Hit
TGAGATGAAGCACTGTAGCTCTAATCTCGT1666950.5227263754536637No Hit
TCCTTCATTCCACCGGAGTCTATCTCGTAT1636770.5132624551134067No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTA1633790.5123279792149983No Hit
TGAGATGAAGCACTGTAGCATCTCGTATGC1628060.5105311513969176No Hit
TGAGATGAAGCACTGTAGCAATCTCGTATG1606960.50391456030416No Hit
CACCCGTAGAACCGACCTTGCATCTCGTAT1590080.49862128742995393No Hit
GTACAGTACTGTGATAACTGAATCTCGTAT1386440.4347633438219368No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTA1364740.4279586032194326No Hit
TGTAAACATCCTCGACTGGAAGCATCTCGT1349560.423198420622842No Hit
TAGCTTATCAGACTGATGTTGACATCTCGT1277540.4006142078029177No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTA1232690.38655003194935467No Hit
TGAGATGAAGCACTGTAGATCTCGTATGCC1219370.38237311283297876No Hit
TACAGTACTGTGATAACTGAATATCTCGTA1200170.3763523285210856No Hit
AACCCGTAGATCCGAACTTGTGAATCTCGT1181500.37049774294280197No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTA1136530.3563959371872896No Hit
TACCCTGTAGAACCGAATTTGATCTCGTAT1000780.31382711060710733No Hit
TAGCACCATCTGAAATCGGTTATCTCGTAT937130.29386758344814895No Hit
TGAGATGAAGCACTGTAGCTCTTATCTCGT935670.2934097529744321No Hit
TGAGATGAAGCACTGTAGAATCTCGTATGC907210.2844851945621154No Hit
CTTTCAGTCGGATGTTTGCAGATCTCGTAT884910.27749230444986445No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTAT851390.266981018505351No Hit
CTTTCAGTCGGATGTTTGCAGCATCTCGTA850710.2667677823943047No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTAT843600.2645382107038068No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTA807230.25313321458799665No Hit
TCCTTCATTCCACCGGAGTCTGTATCTCGT757760.23762028750938433No Hit
TGAGATGAAGCACTGTAGAAAATCTCGTAT755750.23698998665173304No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTA750420.2353185918401502No Hit
TAGCTTATCAGACTGATGTTGATCTCGTAT749430.2350081451490682No Hit
ACCCTGTAGAACCGAATTTGTATCTCGTAT694680.2178395023846853No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGT641150.2010534302901206No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAA637000.19975206284770616Illumina Paired End PCR Primer 2 (95% over 24bp)
TAATACTGCCGGGTAATGATGGAATCTCGT615260.19293477894455213No Hit
TGTAAACATCCTTGACTGGATCTCGTATGC573330.17978626403842288No Hit
TGAGATGAAGCACTGTAGCAAATCTCGTAT560590.17579122278146875No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTA541380.16976730264441314No Hit
TAATACTGCCGGGTAATGATGGATCTCGTA531300.16660639088066923No Hit
GTCCAGTTTTCCCAGGAATCCCTATCTCGT523670.16421375628172416No Hit
TGAGGTAGTAGGTTGTATGGTTATCTCGTA502690.15763479509091588No Hit
TACCCTGTAGAACCGAATTTGTGATCTCGT476530.14943147646596142No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATG475250.14903009084516855No Hit
TCAGTGCACTACAGAACTTTGATCTCGTAT474940.14893288026513277No Hit
TGAGATGAAGCACTGTAGAAATCTCGTATG455510.14283997197450338No Hit
TACAGTACTGTGATAACTGAAATCTCGTAT419170.13144438333418051No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTAT411820.12913955183978393No Hit
TCAGTGCACTACAGAACTTTGAATCTCGTA409180.1283116939968986No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATG392150.12297138374525586No Hit
TACAGTACTGTGATAACTGAAGATCTCGTA369120.11574957839614647No Hit
AACCCGTAGATCCGATCTTGTATCTCGTAT368220.11546735413152646No Hit
ACCCTGTAGAACCGAATTTGTGATCTCGTA348760.10936503836540974No Hit
TGTAAACATCCTCGACTGGAAATCTCGTAT346720.1087253300322711No Hit
TGAGATGAAGCACTGTAGCTCTCTCTCGTA335560.10522574915098318No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTA335100.10508150119351073No Hit
GTCCAGTTTTCCCAGGAATCCCATCTCGTA332000.10410939539315298No Hit
GTCCAGTTTTCCCAGGAATCCATCTCGTAT330640.10368292317106055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)