FastQCFastQC Report
Wed 1 May 2024
EGAF00000829101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22333049
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTA16130367.222641207655972Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1237100.5539324254381925Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA305280.13669427761520606Illumina Paired End Adapter 2 (100% over 32bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTA283200.12680758458014396Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)