FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30585932
Sequences flagged as poor quality0
Sequence length75
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4714311.5413327931285534Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC409400.133852386777032Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG347070.11347373688007938Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)