FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000829111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36108429
Sequences flagged as poor quality0
Sequence length100
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACGGATCTCGTA31029488.593417343080754Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1923460.5326900264755356Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACGGATCTCGGA1580200.4376263503460647Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACGGATATCGTA924270.25597070423639867Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACGGATCTCGCA509460.14109171019320724Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA502620.13919741565051197Illumina Paired End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)