FastQCFastQC Report
Wed 1 May 2024
EGAF00000829129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30241771
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1063280.3515931656251216Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGATGTATCTCGTA789850.2611784871990467Illumina Paired End Sequencing Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)