FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11268093
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1661341.47437547773168Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTAT155080.13762754709248495Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC153500.13622535774243255Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA118940.10555468436407119Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)