FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10614892
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1388251.3078324301368305Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC167100.15742034869502206Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG163330.15386873460417685Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTAT151710.14292184979366723Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT132400.12473042589599592Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)