FastQCFastQC Report
Wed 1 May 2024
EGAF00000829203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28521953
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATCTCGTA4245211.488400881945216Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG478670.16782511351869908Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATATCGTA325290.11404899236738802Illumina Paired End Sequencing Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)