FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14555699
Sequences flagged as poor quality0
Sequence length75
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1162990.7989928893143504Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACCGGAT332210.2282336286288965Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)