FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16614576
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2516141.514417220156566Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACAT699740.42116031128329723Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACA249950.15044019179303764Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC243780.1467265851382545Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA192320.11575378149884777Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)