FastQCFastQC Report
Thu 9 May 2024
EGAF00000829219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29454219
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGCCGATCTCGTA11614093.943098949593605Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1052890.35746661624265097Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGGCCGATATCGTA488180.16574196043018488Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA412270.13996976120806326Illumina Paired End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)