FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15419887
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3600312.334848497917008Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCAT519370.3368182918590778Illumina Paired End PCR Primer 2 (97% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC297540.19295861247232227Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA212290.13767286362085532Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCA179170.1161941069996168Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG164500.10668041860488343Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)