FastQCFastQC Report
Fri 10 May 2024
EGAF00000829223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14641177
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTA7466495.099651482937471Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG562270.3840333328392929Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGGA212970.14545961707859964Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA186050.12707311714078726Illumina Paired End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)