FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14641177
Sequences flagged as poor quality0
Sequence length75
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3360042.2949247864430573Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCAT480120.32792445580024066Illumina Paired End PCR Primer 2 (97% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC379180.25898191108542706Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG364450.24892124451469988Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT290800.19861791166106385Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCA167190.11419163910114603Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)