FastQCFastQC Report
Tue 26 Sep 2023
EGAF00000829225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42876187
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4554191.0621723428904721Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCAT767180.17892915710998275Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC458300.10688916903921516Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG429710.10022113207034944Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)