FastQCFastQC Report
Mon 18 Sep 2023
EGAF00000829227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48070335
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14392182.9939837115759644Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAAT3141520.653525713935632Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG1375800.2862056193284278Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC1373190.2856626649263002Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAA1171300.24366378973643518Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA1165840.24252795409060493Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT1104510.22976956578313837Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA1040150.21638085110078803Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA775880.16140515767156605Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)