FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000829247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32399174
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4734491.4612995997984393Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAAT462530.14275981233348728Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC356120.10991638243616952Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG329010.10154888516602306Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)