FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000829267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31557857
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4092831.2969290024984903Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCCCAT345800.10957651528746074Illumina Paired End PCR Primer 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)