FastQCFastQC Report
Thu 16 May 2024
EGAF00000829269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50198788
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTA9114171.815615548327581Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG918550.18298250547403655Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAACTAATCTCGTA528060.10519377479790946Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)