FastQCFastQC Report
Sun 5 May 2024
EGAF00000829301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40020854
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAATCTCGTA6481721.619585629032304Illumina Paired End PCR Primer 2 (97% over 41bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG674240.16847216703571594Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATGTCAATCTCGTA420210.10499775941812735Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)