FastQCFastQC Report
Thu 9 May 2024
EGAF00000829307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29387702
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAATCTCGTA5264641.7914432370384048Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG502360.1709422533275994Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAATCTCGTA463140.15759653476818297Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)