FastQCFastQC Report
Wed 8 May 2024
EGAF00000829317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12639457
Total Bases947.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA2192481.734631479817527Illumina Paired End PCR Primer 2 (97% over 42bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATATCGTA199950.15819508701995663Illumina Paired End PCR Primer 2 (97% over 42bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA151420.11979945024537052Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)