FastQCFastQC Report
Wed 8 May 2024
EGAF00000829319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12704889
Total Bases952.8 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA3004992.3652233404006915Illumina Paired End PCR Primer 2 (97% over 42bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATCTCGTA211730.1666523808275696Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCACGATATCGTA166650.1311699771639091Illumina Paired End PCR Primer 2 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)