FastQCFastQC Report
Wed 8 May 2024
EGAF00000829331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11269070
Total Bases845.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA5069704.498774078073878Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG770480.6837121430606075Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA222720.19763831443056082Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATATCGTA188670.16742286630573774Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA122740.1089175947970862Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)