FastQCFastQC Report
Wed 8 May 2024
EGAF00000829333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11709710
Total Bases878.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA6720465.73921984404396Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG900160.7687295415514133Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA262220.2239338121951782Illumina Paired End Adapter 2 (100% over 32bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA166350.14206158820329454Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATATCGTA164070.1401144861828346Illumina Paired End Sequencing Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)