FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11709710
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3604523.0782316556088922Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGAT763780.6522620970117963Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG428310.3657733624487711Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC278990.2382552599509296Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGA232590.19863002585034129Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT215810.1843000381734475Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA211530.1806449519245139Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA152730.13043021560738907Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA124370.10621099924763296Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)