FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000829421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30661955
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA8825452.878306357177812Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATAT925280.3017681031754172Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC703590.22946677731410145Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG678610.2213198734392507Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT508440.16582112914848385Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA472130.15397909233119675Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA436820.14246319257855541Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACGATA307630.10032954519697129Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)