FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000829461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801002
Sequences flagged as poor quality0
Sequence length75
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA990180.916748279465183Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATAT266580.24681043480965933Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG153680.14228309558687238Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC112660.104305137615936Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)