FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15766636
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2256611.4312564836278328Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGAT719270.45619750465476594Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG237480.15062185744631892Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA209100.13262182243568Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGA207680.13172118643444294Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC181890.11536386075000399Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA169250.10734693183758412Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)