FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10435411
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1554011.4891699042807227Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTAT321620.3082006065693052Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC126490.12121228382859094Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTA105320.10092558884360185Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)