FastQCFastQC Report
Wed 8 May 2024
EGAF00000829491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10707023
Total Bases803 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTA4118013.846083080236215Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG459940.42956851778500893Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTA196590.18360845960637237Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA143590.13410823905020097Illumina Paired End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)