FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000829495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24115860
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5110512.119148974989903Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCAT867360.35966372337540525Illumina Paired End PCR Primer 2 (97% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC313530.13000987731725097Illumina Paired End PCR Primer 2 (97% over 39bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA307930.1276877540340672Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG273630.11346474892456665Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)