FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000829505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000829505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17464307
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4479832.5651347058889886Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGAT823490.47152744165571525Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA326600.18700999701849033Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC275620.15781903055185642Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG253850.14535360607208747Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGA231300.1324415563698004Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT208780.11954668456068712Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)