FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000834614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000834614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length42
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATGTACAGATCGGAAGAGCGGTTCAGCAGGAATGCCG134890.337225Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCG70410.17602500000000001Illumina Paired End PCR Primer 2 (100% over 42bp)
TACCTCCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG62220.15555Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)