FastQCFastQC Report
Wed 8 May 2024
EGAF00000834623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000834623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases168 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCT1934044.8351Illumina Paired End PCR Primer 2 (97% over 41bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAG327280.8182Illumina Paired End PCR Primer 2 (97% over 36bp)
CTCCCGAGAGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCG302520.7563Illumina Paired End PCR Primer 2 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)