FastQCFastQC Report
Sat 10 Sep 2022
EGAF00000851596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000851596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACTGTATAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1254973.137425Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCT58590.146475Illumina Paired End PCR Primer 2 (97% over 41bp)
AGCGACTGTTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA46080.1152Illumina Paired End PCR Primer 2 (100% over 32bp)
AGCGACTTATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA45970.11492500000000001Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)