FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000851599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000851599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156659
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACTGTATAGATCGGAAGAGCGGTTCAGCAGGAATGCCG388513.3588983442829736Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCT18410.15916532011595466Illumina Paired End PCR Primer 2 (97% over 41bp)
AGCGACTGTTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA14530.12562042918440094Illumina Paired End PCR Primer 2 (100% over 32bp)
AGCGACTTATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA14460.12501523785316157Illumina Paired End PCR Primer 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)