FastQCFastQC Report
Thu 9 May 2024
EGAF00000859589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000859589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271549
Total Bases191.8 Mbp
Sequences flagged as poor quality0
Sequence length35-85
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG61900.272501275561302Illumina Single End PCR Primer 1 (100% over 50bp)
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG30530.13440167920656787No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT29110.1281504383132391No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT27860.12264758541418214No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA24410.10745971141278485No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA23590.10384983991100347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)