FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000860523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29075226
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC17779266.114917215088887TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1694120.582667869890332TruSeq Adapter, Index 4 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC1033870.3555845103319231TruSeq Adapter, Index 4 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG929360.31963981982461637TruSeq Adapter, Index 4 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT745890.2565379887330885TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC688300.2367307480258279TruSeq Adapter, Index 4 (98% over 50bp)
CACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT613360.21095622782089465TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)