FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000860579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34232287
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC14958804.369792763188741TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1591730.4649791584184837TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC1065330.3112062013268351TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT800050.2337121092727459TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT533180.1557535434310889TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC376240.10990793574498835TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)