FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000860581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16554004
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC6533743.9469242607407846TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG843520.5095564795079184TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC607750.3671317223313465TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT443240.2677539524576652TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT332910.20110542440366694TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT242910.14673791307528983TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC221730.13394342540934506TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)