FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000860583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35151334
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC15847174.508269871066629TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1881210.5351745683392841TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC1320470.3756528841835704TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT949930.2702400995649269TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT674430.19186469566133676TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC564500.1605913448405685TruSeq Adapter, Index 9 (98% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT411970.11719896604777502TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)