FastQCFastQC Report
Wed 1 May 2024
EGAF00000860653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26842657
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA6579982.4513147115056455TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT5141951.915589056627293TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT382050.14232942737375068TruSeq Adapter, Index 15 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)