FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000860735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23839256
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC8419753.531884552101794TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG933080.39140483243269003TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC266820.11192463388958111TruSeq Adapter, Index 4 (98% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC264880.11111085010371129TruSeq Adapter, Index 4 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG260610.1093196868224411TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)