FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000860737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12535830
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3713862.9625960147832253TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG460750.36754646481325925TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC162940.12997942697053166TruSeq Adapter, Index 4 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG147930.11800574832300692TruSeq Adapter, Index 4 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC145260.11587585345366042TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)