FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000860739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24524347
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9115313.71684106410662TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1071020.43671703063082573TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC323450.13188934245629455TruSeq Adapter, Index 4 (98% over 50bp)
AGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTG301990.1231388546247531TruSeq Adapter, Index 4 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTC301300.12285750156772778TruSeq Adapter, Index 4 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT250820.10227387501897604TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)