FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000860781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20252536
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC8640454.266354593814818TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1103670.5449539751466187TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC384380.18979351524174554TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC292590.14447079615115854TruSeq Adapter, Index 9 (98% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT270340.13348451769200656TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT237230.11713594781414041TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)