FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000860811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20116350
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2499611.2425763123031763TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2143681.0656406356023833TruSeq Adapter, Index 13 (97% over 40bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC251850.12519666838168952TruSeq Adapter, Index 13 (96% over 31bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG251310.1249282300218479TruSeq Adapter, Index 13 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)