FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000860825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27679410
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA5114441.8477416968063987TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4987581.8019097950425964TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT339890.1227952474420517TruSeq Adapter, Index 15 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)