FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000860945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000860945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28131548
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC14080435.005209809286002TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2086000.7415162507232095TruSeq Adapter, Index 9 (100% over 49bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC1002540.3563756960690539TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT695040.2470678115544868TruSeq Adapter, Index 9 (100% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT686090.24388633003772134TruSeq Adapter, Index 9 (100% over 50bp)
ACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTT516090.18345595485893632TruSeq Adapter, Index 9 (100% over 50bp)
TCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAA319530.11358422224045403TruSeq Adapter, Index 9 (100% over 46bp)
CGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAA303090.10774024948787034TruSeq Adapter, Index 9 (100% over 48bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)